Correlation of miRNA and SILAC protein expression in a primary cancer cell line

摘要

Several proteins were found to be differentially expressed between normal and malignant breast cell types using SILAC and mass spectrometry. MicroRNA microarrays revealed several differentially expressed miRNAs in the same sample set. Differential miRNA expression was confirmed by qRT-PCR. Putative miRNA binding sites were found in the 3'UTRs of the gene mRNAs of the proteins identified by SILAC and mass spectrometry. Initial studies in MCF-7 cells suggested a functional relationship between two miRNA and their putative targets.