Evaluation ofwashing-disinfectant-TADD conditions required to produce a negative PCR result when monitoring the presence of PRRSV on metal surfaces

摘要

Because contaminated livestock trailers are a significant risk for transmitting viruses between herds, various methods of washing, disinfecting, and TADD conditions have been evaluated for their effectiveness in inactivating porcine reproductive and respiratory syndrome virus (PRRSV) on contaminated surfaces. In practice, environmental samples are sometimes tested by PCR for monitoring compliance with trailer sanitation and decontamination protocols. However, information on when to expect negative PCR results after adequate trailer sanitation is lacking. The objective of this study was to evaluate whether there are conditions associated with washing-disinfectant-TADD procedures that will consistently produce a negative PCR result for the purpose ofmonitoring compliance with trailer sanitation and decontamination protocols for PRRSV on metal surfaces.