In Vitro Mineralization and Cell Adhesion on Surface Modified Poly(2-hydroxy ethyl methacrylate-co-methyl methacrylate)

摘要

Poly(2-hydroxyethyl methacrylate-co-methyl methacrylate) HM, was synthesized by free radical copolymerization, cross-linked with ethylene glycol dimethacrylate and phosphorylated. The phosphate coupling was ensured by ATR spectroscopy. The in vitro mineralization ability of the phosphorylated HM (designated as PHM) was investigated by studying the nucleation and growth of calcium phosphate on its surface by immersing in simulated body fluid (SBF) solution. The coating morphology was studied by SEM and the Ca/P ratio of the coating by EDX analysis. The cell adhesion behaviour of PHM was studied by seeding Human osteosarcoma (HOS) cells for one week followed by SEM analysis along with HM as control. It was observed that HOS cells exhibited biomineralization of calcium phosphate on the surface of HM as well as on PHM with a significantly higher amount on the surface of PHM as observed by von kossa staining method. The results show that PHM is capable of in vitro mineralization under simulated physiological condition, promotes cell adhesion by providing an excellent cell friendly surface and it exhibits biomineralization of calcium phosphate in presence of HOS cells.