Development of Nanophase β-Tricalcium Phosphate/Collagen Fiber Composites for Improving Cell Adhesion and Proliferation

摘要

A novel porous beta-tricalcium phosphate /collagen fibers (β-TCP/CF) composite, having a well-dispersed nano-sized β-TCP in collagen matrix, was developed by a wet-chemical method. Cytocompatibility of the nano-composite was evaluated by cell attachment, proliferation, alkaline phosphotatse (AKP) activity and morphology analysis. Conventional β-TCP and only cell well were served as controls. These in vitro assays showed that the β-TCP/CF composite elicited cell adhesion and proliferation better then controls. Moreover human osteoblast-like cell culture showed improved cell growth with the highly characterized nanophase structure. SEM examination also demonstrated that the nano-sized composite exhibited much more viable cells in attachment on the surface compared with the conventional β-TCP. At 1, 3 and 5 days, AKP activity was not significant different between the tested and control groups, while at 7 day after culture, significantly increased AKP activity was observed for β-TCP/CF than for the conventional group. This study confirmed the remarkable improvement of cell adhesion and proliferation of the nano-sized β-TCP/CF composite, which could be a new promising candidate for tissue engineered bone substitute.