Cytotoxicity in cultured RPE: A comparative study between continuous wave and mode-locked lasers

摘要

We have previously shown that minimum visible lesions (MVL) generated in vivo by exposures at threshold energies are not ophthalmoscopically visible at one hour, but become visible 24 hours post-exposure. Our preliminary studies toward understanding this phenomenon at the molecular level are presented. We provide threshold data relating cytotoxicity with energy for acute laser exposures in cultured retinal pigment epithelial cells. Rapid loss of cell viability is used as an initial endpoint of analysis. Comparisons of damage efficiency for continuous wave and mode-locked lasers are presented.