Application of an enzymatic reduction system to ethyl (S)-4-chloro-3-hydroxybutyrate production

摘要

Optically active ethyl 4-chloro-3-hydroxybutyrate (CHBE) is an useful chiral building block applicable to the synthesis of Pharmaceuticals. Shimizu et. al established practical production method for (R)-CHBE (100 g/l) through enzymatic asymmetric reduction of ethyl 4-chloro-3-oxobutanoate (COBE) by the combination of an enzyme from Sporobolomyces salmonicolor and a coenzyme-regeneration system. (S)-CHBE as well as (R)-CHBE is a versatile chiral synthon for drug synthesis. There have been no reports that such high concentration of (S) -CHBE can be obtained by means of enzymatic COBE reduction. In this report, we describe the similar method for (S)-CHBE production by use of an (S) -specific carbonyl reductase from Candida magnoliae.