DNA Sequence Detection Using Surface Enhanced Resonance Raman Spectroscopy (SERRS) in a Homogeneous Multiplexed Assay

摘要

Surface enhanced resonance Raman scattering (SERRS) is an analytical technique with several advantages over competitive techniques in terms of improved sensitivity and multiplexing. We have made great progress in the development of SERRS as a quantitative analytical method, in particular for the detection of DNA. However, the lack of quantitative data relating to real examples has prevented more widespread adoption of the technique. Detection of specific DNA sequences is central to modern molecular biology and also to molecular diagnostics where identification of a particular disease is based on nucleic acid identification. Many methods exist and fluorescence spectroscopy dominates the detection technologies employed with different assay formats. Another advantage of SERRS over existing detection techniques is that of the ability to multiplex which is limited when using techniques such as fluorescence. We have clearly shown the great potential of SERRS for multiplexing and have simultaneously detected five labelled oligonucleotide sequences using dual wavelength excitation (Figure 1)1 and six labels using one excitation source combined with chemometrics to resolve the data.