Preparation and Characterization of Multivalent Macrocyclic Hosts

摘要

@@1.Introduction Protein surface recognition by artificial receptors is an important concept in chemical biology. Numerous attempts have been made to develop artificial receptors based on macrocycles that can recognize and bind to specific protein surface. In particular, histones, a family of basic proteins that organize eukaryotic chromatins, are attractive as target proteins. Histones have high concentrations of the amino acids lysine and arginine on its surface. On these grounds, we previously developed an anionic cyclophane-based resorcinarene tetramer as a histone binding receptor.[1] On the other hand, fluorescence-probe methods are powerful techniques for studying molecular interactions in analytical chemistry, biochemistry, and cell biology. In the course of our ongoing research on histone surface recognition, we became interested in developing fluorescent-appended cyclophane-based resorcinarene oligomers capable of binding and sensing histone surfaces. We report here the design and synthesis of cyclophane-based resorcinarene oligomers bearing fluorophore moieties and histone surface recognition in aqueous medium by fluorescence spectroscopy, with an emphasis on the specificity.