Cell-material interaction brings complicated phenomena and there are still a lot of scientific questions which are waiting to be answered. Samples for examinations were prepared on polished silicon substrates. The sample surface was covered with different biocompatible coatings: diamond like carbon (DLC), titanium (Ti), titanium nitride (TiN), titanium carbonitride Ti(Sn), titanium oxide (TiO_x), silicon doped diamond like carbon (DLC:Si). A physical vapour deposition (hybrid pulsed laser deposition) was chosen for the deposition. Coating thicknesses in the range of 200 nm up to 1 μm were fabricated. Experiments performed in this work considered channels for the cell migration. Using laser ablation, migration channels were formed. The width of each individual channel depended on the biological cell width, i.e. 10-30 μm. In some cases, the subsequent step after channel formation was the deposition of porous coatings using oppositely charged polyelectrolytes. The porous coatings were stabilized by the cross linking chemical reaction using 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide. The smooth muscle cells were deposited on the surface of the samples without porous coating, directly onto the migration channels. Human umbilical endothelium cells were deposited on the top of the synthetic porous coating. The idea was to use the cells as a tool to study how the migration channels influence through the porous coating, mimicking in this way the natural vessel structure basing on the smooth muscle cells and endothelium cells co-culture.
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