Determining optimal transport conditions of in vitro cultured human chondrocytes prepared for autologous reomplantation using flow cytometry

摘要

Re-implantation of in vitro cultured chondrocytes is one of the most promising methods for curing deep aseptic lesions of articular cartilage. After cultivation, the cell suspension is submitted to a department of orthopaedic surgery. Transport conditions should guarantee over 90% viability of the shipped cell quantity after 72 hours. Traditionally, trypan blue exclusion test was performed in determining viability and even apoptosis of cultured cells. However, only a limited number of cells can be counted comparing to the size of the sample that can be inspected by flow cytometer analysis.