Bioglass~circleR surface reactivity: from in vitro to in vivo

摘要

Numerous studies have been conducted using various solutions to simulate the biological environment in an attempt to correlate the surface reaction on Bioglass~circleR and its bioactivity in vitro with in vivo results. In this work, we compared the surface reactivity of Bioglass~circleR in tris buffer, a simulated body fluid (SBF) and a cell culture medium (Dulbecco modified eagle medium, DMEM), respectively. The changes in surface reaction from tris, the simplest solution, to DMEM, a complex medium allows us to predict the in vivo behavior of Bioglass~circleR. It has been found that in tris solution, Bioglass~circleR showed faster surface reaction than in SBF and the DMEM. It is likely that the cell culture medium more accurately reflects the in vivo conditions. In DMEM, the modest pH change, the formed HCA crystals, similar in size to the mineral phase of bone and the gradual HCA growth which allows more cells and growth factors to adsorb or attach to the silica gel layer, provided more evidence for the excellent biocompatibility of Bioglass~circleR.