The response of human osteoblast cell lines to phosphate-based soluble glasses

摘要

Two human osteoblast cell lines, MG63 and HOS (TE85), were incubated in vitro in culture medium containing increasing concentrations of extracts of phosphate-based glasses. The effects of the extracts on the proliferation of the cells were measured using the MTT assay. A cell ELISA assay was used to measure fibronectin (FN), osteonectin (ON) and bone sialoprotein (BSP), antigens which play a fundamental part in connective tissue integrity and function. The results indicated that, over a period of 5 days, the glass extracts did not adversely affect the growth of the cells compared with control cultures. However, the expression of FN and especially ON, but not BSP, was found to be down-regulated, especially when cells were cultured in the presence of the more soluble glass extracts. These findings suggest that these glasses are not cytotoxic and could be effective for use in bone repair systems after their composition is modified to elicit an optimal biological response from the host tissue.