Effect of different macromolecules on viscous and microrheologic properties of blood at various temperatures

摘要

Microrheological parameters of red blood cells (RBCs) and blood viscosity were studied by different optical andrheometric techniques. Measurements were performed under in vitro condition both on the EDTA-stabilized whole bloodsamples at various temperatures and on the RBCs in autologous serum without platelets at different concentrations ofproaggregant macromolecules (Dextran 150, Dextran 500 and albumin). The nonlinear dependences of the aggregationparameters on the concentration of the proaggregant macromolecules in autologous serum were observed. Rheometry ofhuman whole blood samples demonstrates a power law dependence of the suspension viscosity on temperature at suchshear rates that allow the RBC aggregation. We assume that there may be an unaccounted temperature dependentsynergetic effect of plasma proteins/macromolecules on RBC aggregation and interaction and, consequently, on theblood viscosity.