In this work we have explored the live-cell friendly nanoscopy method Multiple Signal Classification Algorithm(MUSICAL) for multi-colour imaging of various organelles and sub-cellular structures in the cardiomyoblastcell line H2c9. We have tested MUSICAL for fast (up to 230Hz), multi-colour time-lapse sequences of varioussub-cellular structures (mitochondria, endoplasmic reticulum, microtubules, endosomes and nuclei) in livingcells using low excitation-light dose. Challenges and opportunities with applying MUSICAL for studying rapidsub-cellular dynamics are discussed.
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