Human Dermal Fibroblasts and Bone-Marrow Mesenchymal Stem Cells properties under Silver and Lithium Condition

摘要

Fibroblasts and mesenchymal stem cells are involved in damaged skin repair. Functional activity of the fibroblasts mesenchymal stem cells depends from environment, including antimicrobial preparations. Silver salts are one of the antimicrobial substances. Lithium salts exhibit not only a stabilizing effect on human mental responses, but also antitumor and autophagic effects. The "Bodyguard" hygienic composition contains both silver and lithium. The aim of the study was to study the effect of bound silver and lithium ions in the "Bodyguard" composition and in salt of silver nitrate and lithium of citric acid on human skin fibroblasts (SFs) and bone-marrow mesenchymal stem cells (MSCs) functions. It has been shown that SFs in the presence of "Bodyguard" reduces proliferative activity and significantly increases care in apoptosis. At the same time, silver, and lithium ions alone or in combination do not affect proliferation, enhance migration, and reduce SFs apoptosis. It has been shown that MSCs proliferation increased under "Bodyguard" condition. Whereas silver ions and lithium ions do not have any effect on proliferation of MSCs, but influenced on myeloperoxidase activity, nitric oxide production, migration, and apoptosis. The more pronounced negative action of the "Bodyguard" is most likely due not to silver and lithium ions, but to the very structure of the carrier to which these ions are immobilized.