Synchronous fluorescence spectroscopy with and without polarization sensitivity for colorectal cancer differentiation

摘要

In the present work the possible benefits of implementation of polarization sensitivity with synchronous fluorescence spectroscopy for differentiation of ex vivo colorectal tissue samples was evaluated. Synchronous fluorescence spectroscopy (SFS) is superior method for fluorescence analysis in comparison with standard fluorescence spectroscopy. Parallel and perpendicular linear fluorescence polarization, in respect to the polarization of the excitation light, was additionally applied with SFS. Same parameters based on the area of the most prominent fluorescence maxima for SFS without polarization, with parallel and perpendicular polarization, were compared. The introduced polarization sensitivity improves the statistically significant difference of some of the evaluated parameter for cancerous and healthy tissues and their potential application as diagnostic criteria.