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Characterization of Bacterial Community of 'Hetao' Strong-Flavor Chinese Liquor Daqus by PCR-SSCP

机译:应用PCR-SSCP表征“河套”强香型白酒的细菌群落

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"Daqu" is a fermentation starter that is used to initiate fermentations for the production of Chinese liquor. The total DNA extracted from Daqu samples were used as a template for PCR with universal primers of 16S rRNA. The amplicons were analyzed using single-strand conformational polymorphism (SSCP). It was observed that the bacterial SSCP profile indicated high diversity. The results showed that Leuconostoc citreum, Weissella cibaria, Acetobacter pasteurianus, and Lactobacillus mindensis were the most dominant species. Based on the SSCP analysis, a few differences in community structure were found between Daqu samples.
机译:“Daqu”是一种发酵起动器,用于发起中国酒的发酵。从Daqu样品中提取的总DNA用作PCR的模板,其具有16S rRNA的通用引物。使用单链构象多态性(SSCP)分析扩增子。观察到细菌SSCP轮廓表明高多样性。结果表明,Leuconostoc Citreum,Weissella cibaria,Acetobacter Vareeurianus和Lactobacillus MeDensis是最占优势的物种。基于SSCP分析,在Daqu样本之间发现了一些社区结构的差异。

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