Resultados preliminares del crecimiento de tejido celular usando un bioreactor con sistema de estimulaci#x00F3;n magn#x00E9;tica

摘要

This article presents the preliminary results obtained from an earlier publication of a design study performed to integrate a cellular culture bioreactor along with an external magnetic stimulation system, which was aimed at creating ideal environmental conditions that allowed for cellular culture and cellular tissue growth to take place in three dimensions, under a sterile environment favorable for the development of bioorganic grafts which were exposed to controlled electromagnetic fields. Cellular tissue was used from cellular lines of Normal Human Skin Fibroblast (NHSF) and was seeded on bio-synthetic scaffolds synthesized from polylactic acid (PLA) and Chitosan mixtures. The cellular tissue covered scaffold was then introduced into the cellular culture bioreactor, subsequently it was filled with cellular culture medium and put under dynamic flow conditions in while at the same time using the external magnetic stimulation system to irradiate the cellular tissue with controlled magnetic fields. The results present static tests of cell growth where the maximum change in growth was after 48 hours when the cells were exposed to a 1 mT, 60 Hz magnetic field; they proliferated at a rate of 52% without Chitosan, 75% with Chitosan and 53% under dynamic flow versus the non-exposed cellular tissue. These results have allowed us to continue our experimentation techniques to evaluate changes in 3-dimensional growth of magnetically stimulated tissues under dynamic flow.