Comparative Evaluation of Paraoxonase and SGOT in Female Cardiac Patient with Normal Subjects

摘要

Cardiac disease is associated with significantly decrease activity of paraoxonase (PON) as compared to the controls. The present project was design for the comparative evaluation of PON and SGOT in female cardiac patents and normal subjects. Other biochemical test low HDL cholesterol HDL-C is an established indicator for coronary heart disease. Also clear cause and effective relationship between LDL oxidation and atherosclerosis was seen. According to the global risk assessment score for cardiovascular risk production age, gender, total and HDL cholesterol, oxidized HDL, diabetes, hypertension and smoking are predictive values for coronary artery disease. So based on such facts the present work was to determine the PON activity in cardiac disease and normal female subjects at the same time it was compared with other biochemical tests. The blood of female cardiac patients as well as control subjects was collected and serum was separated. The serum was used for assaying of enzymes PON and SGOT. Because PON is located on HDL and prevents the oxidation of LDL. So these two parameters were also determined. The female cardiac patients had low activity of PON and HDL as compared to normal subjects. On the other hand the activity of SGOT and LDL was greater in cardiac patients as compared to normal subjects. the activity of PON (55.38±3.07 U mL-1) and HDL (29.70±2.00 mg dL- 1) was lower and activity of SGOT (219.0±1.57 U mL-1) and LDL (164.7±0.88 mg dL-1) was greater in cardiac female patients as compared to normal female subject (223.6±3.02 U mL-1), (49.04 mg dL-1), (19.86 U mL-1) and (122.5±2.17 mg dL-1) respectively in above mentioned biochemical tests. It was concluded from this work that PON and SGOT were inversely correlated and HDL and PON are related to each other. The value of LDL increase as PON activity decrease. Breakthrough work to be presented: PON has recently attracted attention as a protective factor against oxidative modification of LDL and may therefore play role in atherosclerosis. The unearthing of paraoxonase enzyme activity open a probable innovative route in anticipation of heart disease.