Near-Infrared-Irradiation-Induced Transformation of Surface Topography for Sequential Modulation of Macrophage Phenotypes to Match Tissue Healing Process

摘要

In summary, NIR-responsive PCL surfaces were successfully prepared through the crosslinking of PCL macromonomers in the presence of AuNRs. Temporal flat surfaces can be easily programmed into the films and well maintained under the cell culture conditions. Exposure to NIR light could successfully induce the photothermal heating of embedded AuNRs and consequently switch the flat surface topography to microgroove, leading to the elongation of macrophages and the polarization toward M2-like phenotype in vitro and in vivo. The switched phenotypes could recuit more BMSCs. We further studied the mechanism of phenotype modulation by surface topography; the results indicated that Racl and ROCK played an important role to make a connection between the cell shapes and phenotypes.