Gene transfer by circulating plasma bubble flow

摘要

We have succeeded in injection of plasmid to adherent cells which are suspended in the plasma-bubbles laden circulation flow in a chamber. High-speed plasma-bubbles are generated by glass electrode and the air-liquid interface has a stiction force which draws the gene (plasmid) and stick to the air-liquid interface. The circulating flow increased the chance for cells to contact air-liquid interface of bubbles which enclosed plasma or reactive gas. Finally, the high reactive interface enables gene transfer to cells efficiently. This technology of two-dimensional microfluidic chip contributes an option to the high-throughput gene transfer.