Synthesis and Characterization Functionalized Magnetic Nanoparticles for Biomedical Applications

摘要

In this study, Pluronic® F127 (PF127) modified water-dispersible superparamagnetic iron oxide nanoparticles (SPIONs), PF127-PAAIO, was prepared to prevent the recognition from the reticuloendothelial system (RES). A blood-brain-barrier (BBB) penetrating peptide, angiopep-2, was further conjugated to the surface of PF127-PAAIO (ANG-PF127-PAAIO). Their particle diameters and distributions were measured using dynamic light scattering (DLS) and a transmission electron microscope (TEM). FT-IR was used to confirm the successful synthesis of PF127-PAAIO. The cellular viabilities of U87 cells treated with both magnetic nanoparticles (MNP) were ＞ 90%, indicating that they were non-toxic. The cellular internalization of MNPs in U87 cells were acquired using a flow cytometer and visualized using a confocal laser scanning microscope (CLSM). In conclusion, ANG-PF127-PAAIO has the ability to target the tumor section of the brain. It is potential to be used as a diagnostic imaging agent as well as a chemotherapeutic agent specifically for brain diseases.