Fibrin Based Hydrogels to Promote Healing and Limit Infection in Wound Therapy

摘要

Silver sulfadiazine (SSD) is a widely used broad-spectrum antimicrobial agent to treat burn wound infections. We have designed a fibrin-based wound dressing using chitosan for the controlled release of silver sulfadiazine (SSD). This dressing is intended to control infection and may further aid in neovascularization when applied to topical wounds. In this study, we entrapped SSD into chitosan microspheres (CSM) to produce a controlled release of SSD. The SSD-loaded CSM (SSD-CSM) was then impregnated into a PEGylated fibrin gel (SSD-CSM-PEGylated fibrin gel) and used as a wound dressing. We provide evidence that SSD-CSM-PEGylated fibrin gel supports both antimicrobial activity and tube formation by adipose-derived stem cells (ASC) in vitro and blood vessel in-growth in vivo.