Laccase immobilization on D380 macroporous exchange resin by cross-linking with glutaraldehyde

摘要

Macroporous exchange resins have been applied as supports for enzyme immobilization.The supporters were activated by cross-linking with glutaraldehyde,then Laccase was immobilized.Parameters of the immobilization process were optimized.The results of repeated batch showed the optimal immobilization conditions (10% of glutaraldehyde,pH=3,temperature=45 °C).In addition,the biocatalyst also showed good operational stability,maintaining 45% of its initial activity after 10 cycles of oxidation of ABTS.The immobilized laccase had potential for application in the continuous decolonization of textile effluents.