CHARACTERIZATION OF A NEW DEVICE FOR YEAST CELL GROWTH MONITORING

摘要

Impedance cell-based biosensors are playing a key role in today's miniaturized cell cultivation systems thanks to their sensitivity, efficiency and high level of automation. In this work, a micro sensor with an interdigitated electrode structure is used to monitor impedance changes associated with wine yeast growth. The goal is to monitor cell proliferation in presence of alcohol more quickly and objectively with respect to the traditional ethanol resistance assay. In spite of the existing techniques in the field of electrical biosensors, a new approach with solid agar medium is performed. The device shows an increment in impedance magnitude in concomitance with yeast cell growth. This impedance-based biosensor could follow cell growth at different ethanol concentrations, providing a reliable contribution to the common experimental practises.