Use of Orthogonal Matrix Method for the Production of Levansucrase and Biosynthesis of Levan:A Potential Polymer for Novel Drug Delivery Systems

摘要

Polysaccharides are ubiquitous and undoubtedly themost abundant biopolymers on earth. Over the past 25years, the microbial exopolysaccharides has grown inindustrial importance as alternatives to other synthetic andnatural water-soluble polymers in drug delivery.Microbial polysaccharides can be synthesized in twoways viz., 1) direct production of polysaccharides infermentation broth and 2) biotransformation of substrate(monosaccharide or disaccharides) into polysaccharideusing isolated biocatalysts I.e., enzyme. The latterprocess, used in the present study, allows several benefitssuch as controlled synthesis, reusability of enzyme, higheryield, easy separation, economical process, less effluentproblems and many others.Biotransformation of sucrose solution into levanusing the isolated enzyme was studied. Levan waspurified using gel permeation chromatography. More than10 times levan was produced by enzymatic method ascompared to the conventional fermentation method.