A Tat-Masked Polymer Conjugate for Intracellular Targeting

摘要

Poly N-(2-hydroxypropyl methacrylamide) (HPMA)served as a polymer carrier for PEGylated Tatpeptide coupled through a pH sensitive hydrazonebond. Coupling of the peptide to HPMA wasachieved using a novel aldehyde and maleimidefunctional crosslinker. The ability of this crosslinkerto release attached cargo, and the in vitro activity ofthe HPMA-Tat conjugate in cells was assessed.