A SDS/page/western blot/eia protocol for the specific detection of shrimp viral pathogens

摘要

Shrimp viral diseases have seriously impacted the sustainability and economic success of the shrimp aquaculture industry worldwide. Among several of the recent viral pathogens which caused massive mortalities in cultured shrimp were the yellow-head virus (YHV) and the white spot baculovirus (WSBV) (also called the Chinese baculovirus (CBV) or the systemic ectodermal and mesodermal baculovirus (SEMBV). Both YHV and CBV have been reported by our laboratory to be highly pathogenic for Penaeus stylirostris (blue shrimp) and P. vannamei (white shrimp, the two principal penaeid species commercially cultured in Hawaii and the Western Hemisphere (Lu et al. 1997). They are thus potentially a serious problem particularly to the brood stock industry.