Scattering Medium Depth and Cell Monolayer Positioning with Respect to Beam Field Affect Cell Viability

摘要

The purpose of this study was test the cytotoxic effect of scattered radiation generated with penetration of medium depth upon cells placed inside a water phantom with respect to beam field.We tested the viability of A549 cells exposed to photon radiation, both within the beam field or outside of it.Our measurements show that cells placed within the beam field show decreased viability as compared to non-irradiated control ones, and scale of this effect depends of medium depth.Surprisingly was done, that comparing to non-irradiated cells, the cells placed during exposure outside of the beam field showed decreased viability too.This finding suggests that healthy cells lying outside of the beam field might die as a result of irradiation during radiotherapy.