Effects of Time-Varying Magnetic Fields on Transient increase in Intracellular Ca²⁺Concentration of Cultured Cells

摘要

We tested the effects of some kinds of time-varying magnetic fields (0-1.2T) on neurotransmitter-induced transient increases in intracellular Ca²⁺concentration ([Ca²⁺]) of cultured chromaffin and HeLa cells. After these cells were exposed for 2 hours to these magnetic fields, transient increases in [Ca²⁺]i by addition of acetylcholine or histamine were measured. In control cells, after addition of these neurotransmitters [Ca²⁺]i was increased immediately and then decreased with time in both cells. But, addition of these drugs to the magnetic fields exposed cells increased [Ca²⁺]i to a level similar to that for control cells. These results suggest that the transient increases in [Ca²⁺]i were not significantly influenced by the magnetic fields used in this experiment.