Optimization of Producing Enzyme Conditions for Facultative Anaerobic Cellulose Degrading Bacteria in Aerobic and Anaerobic Environments

摘要

A facultative anaerobic cellulose degrading bacteria was isolated from bovine stomach. Its enzyme production conditions under aerobic and anaerobic conditions were compared with orthogonal optimization. Results showed that under CMC-Na 0.6%, NH_4NO_3 0.20%, Tw-80 concentration of 0.025% and ascorbic acid concentration of 0.015%, the optimal yield CMC-enzyme activity can be achieved at 3874.34μmo/ (g·h); optimal incubation times were 96h ( aerobic conditions)or 120h (anaerobic conditions), and the most favorable temperature is 37 ℃. The best initial medium should have pH = 7, with inoculation of 4% (aerobic conditions) or 8% (anaerobic conditions). Under the optimal conditions, the CMC activity in aerobic environment reached 4210.32μmol/ (g·h), while in anaerobic environment 3415.76μmol/ (g·h) was achieved. The enzyme activity under aerobic condition is 23.26% higher than under anaerobic condition.