In vitro gas production technique to evaluate associative effects among lucerne hay, rice straw and maize silage

摘要

In vitro gas technique was used to evaluate the associative effects between lucerne hay and rice straw (Trial 1) and between their mixture and maize silage (Trial 2) in terms of gas production and microbial protein. Significant positive associative effect existed in gas production and microbial protein when lucerne hay and rice straw were mixed. The optimal ratio was 20:80 at which lucerne hay and rice straw had the highest associative effect. Markedly negative associative effects were found when lucerne hay/rice straw (20/80) mixture was mixed with maize silage. The associative effects based on gas production and microbial protein behaved inconsistent.