Construction of a yeast expression vector of Hepatitis C Virus Non-structural 5B gene

摘要

To construct the yeast expressing HCV NS5B protein with C-terminal 21 amino acids truncated and to acquire the protein for screening the anti-HCV drugs targeting the NS5B. The coding region of HCV NS5B-C21 gene was amplified by PCR and was digested by Xho I and Not I , the gene fragment was cloned into plasmid pPICZα. The positive recombinant plasmid was obtained by identification of PCR amplification; it was named pPICZα-NS5B-C21. Emzymic hydrolysis verified that NS5B-C21 gene was correctly cloned to the vector. The successful constructing of yeast expression plasmid containing NS5B-C21 lay a better basis for carrying out further studies on establishing foundation for screening the anti-HCV drugs targeting the NS5B.