Fabrication of SiO_2-Coated Magnetic Nanoparticles for Applications to Protein Separation and Purification

摘要

Coating of γ-Fe_2O_2 nanoparticles with SiO_2 layer by wet chemical synthesis and its applications to protein separation and purification were investigated. The average particle size of γ-Fe_2O_3 core was 20 nm and SiO_2 layer thickness was 5 nm. The band of OH~- radicals on SiO_2 layer was detected between 3600 and 3200 cm~(-1) in wave numbers, which showed that the surface property of coated nanoparticles was similar to that of conventional fumed silica. Finally, the feasibility of γ-Fe_2O_3/SiO_2 nanoparticles for magnetic separation media in various bio processes was discussed in terms of structural and functional properties such as pore structure and magnetic properties.