Comparison of boric acid tolerance in yeast cells transformed with AtRBP47c'-related genes from Arabidopsis thaliana

摘要

In a previous study, we showed that overexpression of AtRBP47c'-related genes increased boric acid tolerance in wild type yeast. AtRBP47c' is an Arabidopsis cDNA encoding a 47 kDa-RNA binding protein. In this study, we analyzed whether overexpression of AtRBP47c'-related genes also confers boric acid tolerance to yeast strain Y01169, which carries a deletion in a boron exporter gene and thus shows a higher internal boric acid concentration. Although a decrease in growth rate was observed in yeast transformed with AtRBP47a and AtRBP47b, all transformants showed a faster growth rate than the control strain in 80 mM boric acid-containing SD medium. Among 6 genes tested, overexpression of AtRBP47c' conferred the highest boric acid tolerance to yeast. These results indicate that overexpression of these genes confers boric acid tolerance not only to wild type yeast bet also the yeast strain Y01169.