A New Detection of Biomarker Molecule of α-Synuclein for Parkinson Disease by Phospholipid Liposome-Immobilized Cantilever Microsensor with Temperature Stabilization

摘要

In this work, for the first time, chronological behavior of α−synuclein (aSyn) as causative agent for Parkinson Disease has been measured by phospholipid liposome-immobilized cantilever microsensor. Our goal is to detect aggregated, not a monomeric, forms of aSyn in its initial stage, as it is most toxic in patient’s cerebrospinal fluid (CSF). The aSyn aggregates in phosphate buffered saline (PBS) were successfully detected compared to CSF of healthy person. With a developed temperature stabilization method at room temperature, which was stabilized within +/- 0.2/ °C, aSyn aggregation was detected down to 100 pM.