Non-Enzymatic Electrochemical Detection Of Glutamate Using Templated Polymer-Based Target Receptors

摘要

We present a novel electrochemical biosensing platform for the detection of neurotransmitter glutamate using templated polymer-based target receptors. Our sensing approach demonstrates, for the first time, a nonenzymatic approach without the need of glutamate oxidase, leading to a more specific and rapid response. The proposed detection principle is based on the following two claims: (1) our templated polymer-based receptor results in specific molecular recognition of the target glutamate and, (2) upon target binding, the polymer undergoes a conformation change which can then be measured via electrochemical techniques. This sensing platform has the potential to provide direct monitoring of a variety of nonelectroactive species and to eliminate the incorporation of enzymes thereby providing a simpler and more robust alternative to enzyme-based sensors.