Patterning of Different Motor Proteins Using Aqueous Two-Phase System

摘要

In this research, we attempted to pattern kinesin-1 and dynein in an in vitro assay aiming to explore the insights of cellular functions like a tug-of-war phenomenon. Here, the selective fixation of motor proteins was accomplished by using aqueous two-phase system (ATPS) made of polyethylene glycol (PEG) and dextran (DEX) polymers. The trivial influence of polymer solutions on the activity of motor proteins ensures the compatibility of using ATPS. Further, the selective fixation of kinesin and dynein was assessed using polarity marked MTs (PMMTs) which display a profound minus and plus end leading motility on patterned kinesin and dynein turf respectively.