Effect of iron oxide nanoparticles on the concentration-versus-sizes relation of proteins in the blood plasma and serum, and in model solutions

摘要

The effect of iron (Ⅲ) oxide nanoparticles produced in acoustoplasma discharge with cavitation on the concentration and the sizes of particles in model protein solutions, human blood serum and plasma samples is studied. Dynamic and static light scattering data on size and concentration of particles show that the nanoparticles addition to fibrinogen-thrombin system affects the course of enzymatic reaction. Interaction of nanoparticles with fibrinogen solution (before thrombin addition) does not significantly change the distribution of scattered light intensity on particle sizes. Comparison of the relations of particle sizes and their concentration for fibrinogen solution with and without nanoparticles shows an increase of the slope of size-concentration relation in a log-log scale, which indicates an increase in the concentration of small particles and decrease of big ones. For model solution of fibrinogen with thrombin, initially incubated with iron oxide nanoparticles, the slopes of the size-concentration relation equals to k = -(4.62±0.33) and slightly differs from the slope of the relation for fibrinogen-thrombin system without nanoparticles k=-(4.23±0.28). We believe that changes in the size-concentration relation indicate the interaction of nanoparticles with proteins, which results in gelation rate change.