Using ERIC-PCR for Monitoring Changes in the Structure of Microbial Community in UASB for Treating Hydrolytic Liquid from Municipal Organic Wastes

摘要

Two upflow anaerobic sludge blankets for treating hydrolytic liquid from municipal organic wastes were adopted to research the changes of microbial community structure with ERIC-PCR technique. In one of them, combination of Fe, Co, Ni with an amount of 5.0 mg/L, 1.0 mg/L, and 1.0 mg/L respectively was added in influent; for the other the combination was not be added in influent. Experimental results indicated that the addition of metal ions did not lead to changes of microbial community structure and dominant microbial categories. It, to some extent, made the proportion of dominant microbes ascend in the reactor. Possible mechanisms of the addition to promote the treatment of hydrolytic liquid include the improvement of the synthesis of dominant microbial cells and enzymes, as well as the activation of the enzyme catalysis in the biological reaction.