Two-photon imaging and selective laser ablation of Cochlea hair cells through a multimode fiber probe

摘要

We demonstrate multimode fiber probe that accomodates dual modality properties for high power ultrashort pulsedelivery. A commercially available multimode graded-index (GRIN) fiber is used for two-photon imaging and/orfemtosecond laser ablation of Cochlea hair cells. Lensless focusing and digital scanning of ultrashort pulses through theoptical fiber is realized using the transmission matrix technique. We investigate the performance and the limitations ofthe GRIN probe in terms of focusing efficiency and peak power delivery. Selective laser ablation guided by the twophotonimage obtained through the GRIN fiber is realized by proximally-only control of the femtosecond laser beam.