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Whole body in vivo examination of small animals by simultaneous X-Rays/optical tomography: comparison between the reconstructions obtained with different types of fluorescent labels

机译:同时进行X射线/光学层析成像的小动物全身检查:使用不同类型的荧光标记获得的重建物之间的比较

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Small animal diffuse optical tomography is an appealing tool for the investigation of molecular events in cancer research and drug developments. The combination of the functional information brought by an optical system and the anatomical information delivered by X-Rays enables ⅰ) a fast multimodality animal examination; ⅱ) the correlation between the biodistribution of the molecular probes and the morphology of the animal; ⅲ) a more accurate optical data reconstructions by using the anatomy of the animal as a constrain in the reconstructions. A small animal multimodality tomographer for the coregistration of fluorescence optical signals and X-rays measurements is used in the present study. The optical system is composed with a CW laser and a CCD camera coupled with an appropriate combination of filters for the fluorescence detection. The animal is placed inside a transparent tube filled with an index matching fluid. The X-ray generator and detector have been positioned perpendicularly to the optical chain. Original optical calibration techniques have been developed in order to control at any time the alignment between the incident beam, the axis of the cylinder and the focus plan of the CCD. Specific developments have also been handled for obtaining the geometry correlation between optical and X-rays data reconstructions. This experimental setup is used in the present work for a study conducted on different kinds of fluorochromes for the purpose of the development of new molecular probes. The instrument is also used for in vivo biological study conducted on mice bearing tumors in the lungs, and tagged with near infrared optical probes (targeting probes such as Transferin-AlexaFluor 750 or such as RAFT-(cRGD)_4-Alexa700/Alexa750).
机译:小动物漫射光学层析成像技术是用于研究癌症研究和药物开发中的分子事件的诱人工具。光学系统带来的功能信息与X射线提供的解剖信息相结合,使得:ⅰ)快速的多模态动物检查; ⅱ)分子探针的生物分布与动物形态之间的相关性; ⅲ)通过以动物的解剖结构为约束条件,进行更精确的光学数据重建。在本研究中使用了一个小动物多峰层析成像仪,用于对荧光信号和X射线测量进行配准。光学系统由CW激光器和CCD照相机组成,并结合了适当的滤光片组合用于荧光检测。将动物放置在装有折射率匹配液的透明管内。 X射线发生器和检测器已垂直于光学链放置。已经开发出原始的光学校准技术,以便随时控制入射光束,圆柱轴和CCD聚焦计划之间的对准。为了获得光学和X射线数据重建之间的几何相关性,还进行了特定的开发。为了开发新的分子探针,在本工作中使用该实验装置对不同种类的荧光染料进行了研究。该仪器还用于在肺部携带肿瘤的小鼠上进行的体内生物学研究,并用近红外光学探针(靶向探针,如Transferin-AlexaFluor 750或RAFT-(cRGD)_4-Alexa700 / Alexa750)进行标记。

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