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Cross-reactive Fluroescent Indicators for Analysis of Metal-ion Mixtures

机译:交叉反应荧光指示剂,用于分析金属离子混合物

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摘要

The conventional approach to optical sensing of metal ions has been to use metal-bionding indicators containing seelctive binding sites linked to a chromogenic or fluorescent tag. The one analyte-one sensor approach tends to be tedious and time consuming, especially where multiple ions need tob e determined. Most metallofluorescent indicators lack sufficient binding discrimination between metal ions to be effective for analysis of mixtures. In this paper we present an approach which exploits the cross-reactivity of semi-selective metallofluorescent indicators, combiend with multivariate analysis, to model a binding pattern for multiple metal ions. Mixtures of metal ions have different cross reactive bindign patterns with each metallofluorescent indicator. A pattern recognition algorithm capable of modeling these bindign patterns allows concentrations of individual metal ions to be determined. The fluorescence responses of the indicators and formation of the metal complexes are pH dependent, therefore calibration was performed at different pH values in the 4-9 pH range. The application of these semi-selective indicators to the development of optical array sensors for continuous, on-site monitoring in multicomponent media is discussed as a useful alternative to the one analyte-one sensor paradigm.
机译:对金属离子进行光学感测的常规方法是使用金属结合指示剂,该指示剂包含与发色或荧光标签连接的渗透性结合位点。一种分析物一传感器的方法往往很繁琐且耗时,特别是在需要确定多个离子的情况下。大多数金属荧光指示剂在金属离子之间缺乏足够的结合辨别力,无法有效地分析混合物。在本文中,我们提出了一种方法,该方法利用半选择金属荧光指示剂的交叉反应性,并与多变量分析相结合,为多种金属离子的结合模式建模。金属离子的混合物与每种金属荧光指示剂具有不同的交叉反应性粘结性图案。能够对这些约束模式进行建模的模式识别算法可以确定各个金属离子的浓度。指示剂的荧光响应和金属络合物的形成均取决于pH,因此在4-9 pH范围内的不同pH值下进行校准。这些半选择指标在光学阵列传感器开发中的应用,用于在多组分介质中进行连续,现场监测,作为一种分析物-一种传感器范例的有用替代方法进行了讨论。

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