Simultaneous Determination of Ciprofloxacin,Enrofloxacin and Florfenicol in Animal Wastewater and Soil Using LC-MS/MS

摘要

In order to predict the main species of veterinary drugs in the environment,a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method is described in this paper.The method uses the reversed-phase column of C8 column,the HLB solid phase extraction cartridge,the 0.1％ formic acid acetonitrile-water solution mobile phase with gradient elution to determine ciprofloxacin (CPFX),enrofloxacin (ERFX) and florfenicol (FF) in animal wastewater and soil.The flow rate was 0.30 mL/min.The detection mode was multiple reaction monitoring (MRM) with positive ionization mode for CPFX and ERFX and negative ion mode for FF,respectively.The injection volume was 10 μL.The quantification limit of the method (MQL) was 20ng/L for FF and 40ng/L for CPFX and ERFX in animal wastewater samples.In soil samples,MQL was 40μg/kg for FF and 50μg/kg for CPFX and ERFX.The correlation coefficient was higher than 0.99.Recoveries of CPFX,ERFX and FF at fortified levels were higher than 67％,with relative standard deviation of 0.72-5.81％.