Label-free Infrared spectroscopic imaging for characterization of necrotic tissue areas on cutaneous squamous cell carcinoma

摘要

In this study, FTIR spectroscopy was used to evaluate the overall biochemical status of necrotic tissue areas of cutaneoussquamous cell carcinoma chemically-induced on mice. FTIR hyperspectral image collected from specimen showed highcorrelation with the photomicrograph obtained by light microscopy, in which we were able to identify clusters associatedto keratin, necrosis and regions with no tissue. Alterations in the protein content were documented in the necrotic tissueareas, indicating changes on protein conformation.