Novel Reducible L-Lysine Copolymers for Nonviral Gene Delivery

摘要

A novel reducible linear L-lysine modified copolymer (LLC) was designed and synthesized as an alternative to high molecular weight non-degradable poly(L-lysine) (PLL) for nonviral gene delivery. The transfection efficiencies of the polyplexes as determined with a luciferase assay showed that LLC polyplexes produced five times higher transfection efficiencies in HDF cells, three times higher transfection efficiencies in MCF-7 cells, and four times higher transfection efficiencies in MA cells as compared to the optimal PLL control. The LLC/pDNA polyplexes showed significantly lower cytotoxicities as compared to the control in HDF, MCF-7, and MA cells at various N/P ratios. Therefore, LLCs are efficient, reducible, and biocompatible polymers for nonviral gene delivery.