Application of Color Digital Holographic Microscopy for Analysis of Stained Tissue Sections

摘要

The analysis of stained tissue sections represents an important tool in medical diagnostics. Color digital holographic microscopy offers subsequent multi-focus true color imaging with simultaneous quantitative phase contrast analysis. Investigations on digital recording and numerical reconstructions of color digital holographic images have been performed by applying a transmission microscope experimental setup in holographic off-axis geometry. Three monochromatic digital holograms with different wavelengths in red, green and blue spectral range are recorded. After digital holographic refo-cusing and compensation for aberrations of the microscope imaging system by digital image correlation the numerically reconstructed amplitude distributions are combined into color images. The applicability of the method is demonstrated by results obtained from stained intestine tissue sections.