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【24h】 Development and testing of multi-well plates absorbance reader for clinical analysis using inexpensive Web-Cam

机译使用便宜的Web-Cam开发和测试用于临床分析的多孔板吸光度读取器

【摘要】Biochemical analysis and clinical tests like glucose, hemoglobin, cholesterol, iron, etc. are crucial for early illness diagnosis like diabetes, anemia and coronary deceases. These tests usually are done in state of the art instruments in well equipped laboratories in health centers. In some cases, these instruments are not portable, so they are not recommended for clinical field studies in remote areas. The present work shows a portable low-cost prototype of multi-well plates reader designed for clinical analysis. A Light Emission Diodes (LEDs) array is used as excitation source and an inexpensive webcam as detector. The light source illuminates the 96 well plates and the webcam take the image with 640 × 480 pixels. The data is acquired and processed by using a portable computer. 96 samples can be read including blanks and calibration standards simultaneously. Light absorption data are processed using a MatLab software designed in our laboratory to obtain calibration curves, standards lectures and samples concentration. The system was evaluated using different analytes series solutions: Neutral Red, Cooper (Ⅱ) Ammonia Complex and Methyl Orange. The results shows that it is possible to measure few micro liters of solutions with adequate exactitude and precision of less than 3%. As possible analytical clinical application, iron determination was performed using Fe(Ⅲ) Thiocyanate complex. This method is usually applied in serum samples analysis. The sensibility achieved with the proposed instrumentation configurations allows the analysis of iron in serum samples in the references values normal range (0.75 - 1.5 mg/L) in human.

【摘要机译】生化分析和临床测试(如葡萄糖,血红蛋白,胆固醇,铁等)对于早期疾病诊断(如糖尿病,贫血和冠心病)至关重要。这些测试通常是在卫生中心设备完善的实验室中使用最先进的仪器进行的。在某些情况下,这些仪器不是便携式的,因此不建议在偏远地区进行临床现场研究。目前的工作显示了便携式多孔板阅读器的低成本原型,旨在用于临床分析。发光二极管(LED)阵列用作激发源,廉价的网络摄像头用作检测器。光源照亮96个孔板,网络摄像头以640×480像素拍摄图像。通过使用便携式计算机来获取和处理数据。可以同时读取96个样品,包括空白和校准标准品。使用我们实验室设计的MatLab软件处理光吸收数据,以获得校准曲线,标准讲座和样品浓度。该系统使用不同的分析物系列溶液进行评估:中性红,库珀(Ⅱ)氨络合物和甲基橙。结果表明,可以以足够的精确度和不到3%的精度测量少量微升溶液。作为临床可能的分析方法,使用硫氰酸铁(Ⅲ)配合物进行铁的测定。该方法通常用于血清样品分析。所提出的仪器配置所实现的灵敏度允许在人体的参考值正常范围(0.75-1.5 mg / L)中分析血清样品中的铁。

【原文格式】PDF

机译 分光光度法;比色法;生物测定法; CCD相机;
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