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【24h】 Tracking SERS-active nanoprobe intracellular uptake for chemical and biological sensing

机译跟踪SERS活性纳米探针的细胞内摄取,以进行化学和生物传感

【摘要】A critical aspect of the use of nanoprobes for intracellular studies in chemical and biological sensing involves a fundamental understanding of their uptake and trajectory in cells. In this study, we describe experiments using surface-enhanced Raman scattering (SERS) spectroscopy and mapping to track cellular uptake of plasmonics-active labeled nanoparticles. Three different Raman-active labels with positive, negative, and neutral charges were conjugated to silver colloidal nanoparticles with the aim of spatially and temporally profiling intracellular delivery and tracking of nanoprobes during uptake in single mammalian cells. 1-D Raman spectra and 2-D Raman mapping are used to identify and locate the probes via their SERS signal intensities. Because Raman spectroscopy is very specific for identification of chemical and molecular signatures, the development of functionalized plasmonics-active nanoprobes capable of exploring intracellular spaces and processes has the ability to provide specific information on the effects of biological and chemical pollutants in the intracellular environment. The results indicate that this technique will allow study of when, where, and how these substances affect cells and living organisms.

【摘要机译】在化学和生物传感中将纳米探针用于细胞内研究的一个关键方面涉及对它们在细胞中的吸收和轨迹的基本了解。在这项研究中,我们描述了使用表面增强拉曼散射(SERS)光谱和映射来追踪等离子活性标记纳米颗粒的细胞摄取的实验。将三种具有正,负和中性电荷的拉曼活性标记物与银胶体纳米颗粒偶联,目的是在单个哺乳动物细胞中摄取时在空间和时间上分析细胞内传递并跟踪纳米探针。一维拉曼光谱和二维拉曼映射用于通过SERS信号强度识别和定位探针。由于拉曼光谱法非常适用于鉴定化学和分子标记,因此能够探索细胞内空间和过程的功能化等离激元活性纳米探针的开发具有提供有关细胞内环境中生物和化学污染物影响的特定信息的能力。结果表明,该技术将允许研究这些物质何时,何地以及如何影响细胞和活生物体。

【原文格式】PDF

机译 纳米探针;细胞吸收;表面增强拉曼散射;拉曼光谱;纳米粒子;等离子体;拉曼成像;
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