Spray-jet assembly interface for the coupling of reversed-phase narrow-bore LC and FTIR spectrometry

摘要

Abstract: Reversed phase liquid chromatography (RPLC) has been coupled to Fourier transform infrared spectrometry (FTIR) via solvent elimination and effluent deposition using a spray jet assembly. The chromatographic separation is hardly affected. Identification limits are in the 10 - 20 ng range. The system can handle eluent containing up to 40% water. The usefulness of the interface in impurity profiling of steroids is demonstrated. !5